diff --git a/.gitignore b/.gitignore
index 5b83825..b5e22d7 100644
--- a/.gitignore
+++ b/.gitignore
@@ -3,3 +3,8 @@
 .vscode
 .idea
 .rumdl_cache
+
+# Native launcher regression application
+/tests/threading/build/
+/tests/threading/logs/
+/tests/threading/.briefcase/
diff --git a/tests/threading/README.md b/tests/threading/README.md
new file mode 100644
index 0000000..88d43cc
--- /dev/null
+++ b/tests/threading/README.md
@@ -0,0 +1,36 @@
+# Native launcher threading regression
+
+This application returns from Python into `UIApplicationMain`, then starts a Python worker while UIKit is otherwise idle. The worker sleeps for 0.1 seconds and schedules a callback on the main thread. The test fails if the worker and callback cannot complete within one second. A three-second watchdog bounds a failure; there is no heartbeat before the verdict.
+
+By default, the test uses released Rubicon 0.5.7 with a lifecycle that does not nest a run loop. It checks that the launcher exposes `sys._ios_app_main_releases_gil = True` before importing application code and actually releases the GIL while UIKit runs. The old launcher fails this test.
+
+From this directory, with Briefcase installed and an iOS Simulator available:
+
+```console
+briefcase run iOS --test --no-input -C 'template="../.."'
+```
+
+Use a fresh generated application after changing the template. Briefcase's `--update` option updates Python application code; it does not regenerate `main.m`. Use `-d <simulator-UDID>` to select a simulator.
+
+## Partial-upgrade compatibility
+
+`--lifecycle default` exercises the unmodified `iOSLifecycle()` constructor used by Toga. The test observes whether the real lifecycle start call returns, checks the advertised capability, and verifies prompt worker delivery on the main thread.
+
+Run each of these combinations, using Rubicon 0.5.7 for the old version and a wheel from the companion Rubicon PR for the new version:
+
+| Rubicon | Launcher | `--expect-nested` | `--expect-capability` |
+| --- | --- | --- | --- |
+| Old | Old | `yes` | `no` |
+| Old | New | `yes` | `yes` |
+| New | Old | `yes` | `no` |
+| New | New | `no` | `yes` |
+
+For example, with a fresh project generated from the new launcher and a local new Rubicon wheel:
+
+```console
+briefcase run iOS --test --no-input -r -C 'template="../.."' -C 'requires=["rubicon-objc @ file:///absolute/path/to/rubicon.whl"]' -- --lifecycle default --expect-nested no --expect-capability yes
+```
+
+Replace the template path with a checkout of the old template for old-launcher cases. Replace the requirement with `rubicon-objc==0.5.7` for old-Rubicon cases. Keep the same test source in all four runs. Changing only the Rubicon requirement can reuse a generated project with `-r`; changing the native launcher requires regenerating and rebuilding it.
+
+All four combinations must deliver callbacks promptly. Only new Rubicon with the new launcher should avoid nesting. No Toga change or application-level capability assignment is involved.
diff --git a/tests/threading/pyproject.toml b/tests/threading/pyproject.toml
new file mode 100644
index 0000000..3f29801
--- /dev/null
+++ b/tests/threading/pyproject.toml
@@ -0,0 +1,21 @@
+[project]
+name = "threading-probe"
+version = "0.0.1"
+description = "Regression test for Python threads while UIKit is idle"
+license = "BSD-3-Clause"
+license-files = ["../../LICENSE"]
+authors = [{name = "BeeWare"}]
+
+[tool.briefcase]
+project_name = "threading-probe"
+bundle = "org.beeware.tests"
+
+[tool.briefcase.app.threadingprobe]
+formal_name = "Threading Probe"
+description = "Regression test for the native launcher GIL"
+sources = ["src/threadingprobe"]
+test_sources = ["tests"]
+requires = ["rubicon-objc==0.5.7"]
+
+[tool.briefcase.app.threadingprobe.iOS]
+supported = true
diff --git a/tests/threading/src/threadingprobe/__init__.py b/tests/threading/src/threadingprobe/__init__.py
new file mode 100644
index 0000000..e69de29
diff --git a/tests/threading/src/threadingprobe/__main__.py b/tests/threading/src/threadingprobe/__main__.py
new file mode 100644
index 0000000..d43cbfb
--- /dev/null
+++ b/tests/threading/src/threadingprobe/__main__.py
@@ -0,0 +1 @@
+raise RuntimeError("Run this application with briefcase run iOS --test")
diff --git a/tests/threading/tests/__init__.py b/tests/threading/tests/__init__.py
new file mode 100644
index 0000000..e69de29
diff --git a/tests/threading/tests/threadingprobe.py b/tests/threading/tests/threadingprobe.py
new file mode 100644
index 0000000..6967dea
--- /dev/null
+++ b/tests/threading/tests/threadingprobe.py
@@ -0,0 +1,109 @@
+"""Check worker delivery and lifecycle selection under a real UIKit host."""
+
+import argparse
+import json
+import sys
+import threading
+import time
+from importlib.metadata import version
+
+from rubicon.objc import ObjCClass, objc_method
+from rubicon.objc.eventloop import CFLifecycle, RubiconEventLoop, iOSLifecycle
+
+
+class NativeLifecycle(CFLifecycle):
+    def start(self):
+        # UIApplicationMain starts the outer run loop after this module returns.
+        pass
+
+
+parser = argparse.ArgumentParser()
+parser.add_argument("--lifecycle", choices=["native", "default"], default="native")
+parser.add_argument("--expect-nested", choices=["yes", "no"], default="no")
+parser.add_argument("--expect-capability", choices=["yes", "no"], default="yes")
+args = parser.parse_args()
+
+# Capture the launcher's flag before the app has a chance to change it.
+capability = getattr(sys, "_ios_app_main_releases_gil", None)
+main_thread = threading.get_ident()
+loop = RubiconEventLoop()
+result = None
+lifecycle_started = False
+lifecycle_returned = False
+lifecycle = iOSLifecycle() if args.lifecycle == "default" else NativeLifecycle()
+original_start = lifecycle.start
+
+
+def observed_start():
+    global lifecycle_started, lifecycle_returned
+    lifecycle_started = True
+    original_start()
+    lifecycle_returned = True
+
+
+# Observe the real start call: a nested NSRunLoop does not return during the test.
+# Do not override the constructor arguments or the launcher's capability flag.
+lifecycle.start = observed_start
+
+
+def report():
+    print(f">>>>>>>>>> EXIT {result} <<<<<<<<<<", flush=True)
+    # Repeat only after the verdict, so logging startup cannot lose the result.
+    loop.call_later(1, report)
+
+
+def finish(returncode):
+    global result
+    if result is None:
+        result = returncode
+        report()
+
+
+def start_probe():
+    started = time.monotonic()
+
+    def delivered():
+        elapsed = time.monotonic() - started
+        nested = not lifecycle_returned
+        passed = (
+            lifecycle_started
+            and elapsed < 1
+            and threading.get_ident() == main_thread
+            and nested == (args.expect_nested == "yes")
+            and (capability is True) == (args.expect_capability == "yes")
+        )
+        print(
+            json.dumps({
+                "rubicon": version("rubicon-objc"),
+                "capability": capability,
+                "nested": nested,
+                "elapsed": elapsed,
+                "passed": passed,
+            }),
+            flush=True,
+        )
+        finish(0 if passed else 1)
+
+    def watchdog():
+        print("Worker callback did not complete before the watchdog", flush=True)
+        finish(1)
+
+    def worker():
+        time.sleep(0.1)
+        loop.call_soon_threadsafe(delivered)
+
+    # There are no intervening main-thread timers to release the GIL by accident.
+    loop.call_later(3, watchdog)
+    threading.Thread(target=worker, daemon=True).start()
+
+
+class PythonAppDelegate(ObjCClass("UIResponder")):
+    @objc_method
+    def application_didFinishLaunchingWithOptions_(
+        self, application, launch_options
+    ) -> bool:
+        loop.call_later(0.1, start_probe)
+        return True
+
+
+loop.run_forever_cooperatively(lifecycle=lifecycle)
diff --git a/{{ cookiecutter.format }}/{{ cookiecutter.class_name }}/main.m b/{{ cookiecutter.format }}/{{ cookiecutter.class_name }}/main.m
index 1acb2f7..1ba53b5 100644
--- a/{{ cookiecutter.format }}/{{ cookiecutter.class_name }}/main.m	
+++ b/{{ cookiecutter.format }}/{{ cookiecutter.class_name }}/main.m	
@@ -167,6 +167,14 @@ int main(int argc, char *argv[]) {
         }
 
         @try {
+            // Advertise the launcher's GIL contract before importing app code.
+            // Rubicon can then let UIKit own the run loop without nesting.
+            // PySys_SetObject keeps its own reference; Py_True is not stolen.
+            if (PySys_SetObject("_ios_app_main_releases_gil", Py_True) < 0) {
+                crash_dialog(@"Could not advertise the UIKit GIL capability");
+                exit(-16);
+            }
+
             // Adding the app_packages as site directory.
             //
             // This adds app_packages to sys.path and executes any .pth
@@ -299,7 +307,17 @@ int main(int argc, char *argv[]) {
                 // [1] http://beeware.org/rubicon
                 // [2] http://pyobjus.readthedocs.org/
                 // [3] https://pythonhosted.org/pyobjc/
-                UIApplicationMain(argc, argv, nil, @"PythonAppDelegate");
+                // UIKit owns the native event loop. Detach Python's thread
+                // state so background threads and native callbacks can acquire
+                // the GIL while the application is idle. Restore it before
+                // exception handling, garbage collection, or finalization.
+                PyThreadState *thread_state = PyEval_SaveThread();
+                @try {
+                    UIApplicationMain(argc, argv, nil, @"PythonAppDelegate");
+                }
+                @finally {
+                    PyEval_RestoreThread(thread_state);
+                }
             }
         }
         @catch (NSException *exception) {