Batch normalize and compile plot profile outputs from Fiji
- Trace plot profile on Fiji and save data in individual folder
- Run batchPlotProfileNormalization script which will ask for desired directory, then iterate through each folder within the directory, opening the plot profile results file, normalizing data and compiling normalized y axis data onto a new excel sheet, saved in the same directory. Additionally, the 95% of the normalized data for each individual folder is calculated.
- Now you have a compilation of normalized signal/pixel intensity with the same amount of X values and a compilation of the 95% for individual files
Y values are normalized by subtracting minimum Y value from all Y-values then dividing by the range of Y (difference between the maximum and minimum values of Y). The same process is down for X. Then interpolation is performed to ensure both X and Y data points have exactly 100 points
batchPlotProfileNormalization1Condition is for 1 condition of data, expected to be in 1 folder batchPlotProfileNormalizationAllConditions is for all conditions of data (expected to be in different folders)